X-ray studies on triclinic crystals of fatty acid binding protein
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چکیده
منابع مشابه
Studies on fatty-acid-binding proteins. The purification of rat liver fatty-acid-binding protein and the role of cysteine-69 in fatty acid binding.
1. A new, simple and high-yield procedure is described for the purification of hepatic fatty-acid-binding protein from rat liver using naphthylaminodecyl-agarose as an affinity column. 2. Cysteine-69 is shown to react slowly, but quantitatively, with 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB), indicating that the thiol group is free, but may be buried within the protein. 3. Fatty acids do not ...
متن کاملCrystallographic studies of protein denaturation and renaturation. 1. Effects of denaturants on volume and X-ray pattern of cross-linked triclinic lysozyme crystals.
Triclinic crystals of hen egg-white lysozyme cross-linked with glutaraldehyde have been treated with various denaturants and found to be susceptible to x-ray structure analysis even after major conformational changes in the protein. Cross-linked crystals were isomorphous with the native form, and electron density difference maps indicated the locations of intermolecular corss-links, but showed ...
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The X-ray study of protein crystals is a difficult and highly specialized field. Eventually, when the structure of a few proteins has been unraveled, the results will be of vital interest to ensytiologists, since they should give information about the spatial arrangement of the “active site” of the enzyme. But meanwhile X-ray methods will have only a secondary interest for enzymologists. The eq...
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iomarkers have come to play an increasingly important ole in the evaluation and management of patients with uspected acute coronary syndromes (ACS). To date, cariac troponins T and I remain the best established biomarers in ACS for both diagnosis and risk assessment. In ddition to providing high sensitivity and specificity for etecting myocardial necrosis, troponins provide assessment f the ris...
متن کاملAdipocyte lipid-binding protein complexed with arachidonic acid. Titration calorimetry and X-ray crystallographic studies.
The association of the adipocyte lipid-binding protein (ALBP) with arachidonic acid (all cis, 20:4 delta 5,8,11,14) and oleic acid (cis, 18:1 delta 9) has been examined by titration calorimentry. In addition, the crystal structure of ALBP with bound arachidonic acid has also been obtained. Crystallographic analysis of the arachidonic acid.ALBP complex along with the previously reported oleic ac...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1985
ISSN: 0014-5793
DOI: 10.1016/0014-5793(85)80603-7